Recently, I have read two papers from one team and published in Science Advance and Signal Transduction and Targeted Therapy. one is named Single-cell RNA-sequencing reveals radio chemotherapy-induced innate immune activation and MHC-II upregulation in cervical cancer. The other is called Single-cell dissection of cellular and molecular features underlying human cervical squamous cell carcinoma initiation and progression*. As we can see, these two papers study cervical squamous cell carcinoma (CESC) and cervical cancer (CC), both of which are based on single-cell sequencing technology.

From research ideas, they are similar, and both apply comparison to different conditions. For CESC, they sampled 13 human cervical tissues at various stages of malignancy. For CC, they tested pre-RCT and post-RCT tumor biopsies from patients with cervical cancer and adjacent normal cervical tissues. I think this is an excellent performance of measurement of variables on the experiment design.

On the analysis methods, they focus on the comparison between pre-condition and post-condition, such as, to test the percentage of epithelia. Most importantly, these two papers' logic is constant or not diverged, which is what I have learned.